IKK alpha Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: IKK alpha Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB5508
• Supplier Catalog Number: orb5508
• Alternative Catalog Number: BYT-ORB5508-100,BYT-ORB5508-200,BYT-ORB5508-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human IKK alpha (551-650/745aa)
• Conjugation: Unconjugated
• Alternative Names: I Kappa B Kinase alpha, IKKalpha, IKK alpha, IkappaB kinase, IkB kinase alpha subunit, IKBKA, IKK 1, IKK A, IKK a kinase, IKK1, IKKA, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells, Inhibitor Of Nuclear Factor Kappa B Kinase alpha Subunit, NFKBIKA, Nuclear Factor Kappa B Inhibitor Kinase alpha, Nuclear factor NF kappa B inhibitor kinase alpha, Nuclear factor NFkappaB inhibitor kinase alpha, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor, TCF16, CHUK1, CHUK, Conserved Helix Loop Helix Ubiquitous Kinase, Conserved helix loop ubiquitous kinase, I Kappa B Kinase 1, IkappaB kinase, IKKA_HUMAN.

Product Description

IKK alpha Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 85 kDa
• UniProt: O15111
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CHUK

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

Images

Blank control (black line): U251. Primary Antibody (green line): Rabbit Anti-IKK alpha antibody (orb5508), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (IKK alpha) polyclonal Antibody, Unconjugated (orb5508) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK alpha CHUK) Polyclonal Antibody, Unconjugated (orb5508) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK alpha CHUK) Polyclonal Antibody, Unconjugated (orb5508) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK alpha CHUK) Polyclonal Antibody, Unconjugated (orb5508) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK alpha CHUK) Polyclonal Antibody, Unconjugated (orb5508) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (IKK alpha CHUK) Polyclonal Antibody, Unconjugated (orb5508) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: Rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-IKK alpha Polyclonal Antibody, Unconjugated (orb5508) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.