Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody, Clone: [2H5], Unconjugated

Product Overview

• Article Name: Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody, Clone: [2H5], Unconjugated
• Biozol Catalog Number: BYT-ORB559074
• Supplier Catalog Number: orb559074
• Alternative Catalog Number: BYT-ORB559074-100,BYT-ORB559074-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthesized peptide derived from human Cytokeratin 5 (550-590aa)
• Conjugation: Unconjugated
• Alternative Names: Active () Monoclonal Antibody Primary Antibody Unconjugated Rabbit Cytokeratin 5 Unmodified WB(1:1000-2000)ICC(1:50-200), IHC(1:50-200),

Product Description

Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [2H5]
• Molecular Weight: 64 kDa
• UniProt: P13647
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: KRT5

Application Notes

• Application Dilute: WB=1:1000-5000, IHC-P=1:500-2000, IHC-F=1:200-1000, ICC/IF=1:100-500, IF=1:200-1000

Images

Paraformaldehyde-fixed, paraffin embedded (Human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Monoclonal Antibody, Unconjugated (orb559074) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse trachea), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 5) Polyclonal Antibody, Unconjugated (orb559074) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: human A431 cell lysates, Primary: Anti-Cytokeratin 5 (orb559074) at 1/500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1/5000 dilution, Predicted band size: 64 kD, Observed band size: 62 kD.

IHC-P analysis of Human skin cancer using Cytokeratin 5 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse trachea using Cytokeratin 5 Polyclonal Antibody at 1:200 dilution.