Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody, Clone: [2F9], Unconjugated

Product Overview

• Article Name: Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody, Clone: [2F9], Unconjugated
• Biozol Catalog Number: BYT-ORB559075
• Supplier Catalog Number: orb559075
• Alternative Catalog Number: BYT-ORB559075-100,BYT-ORB559075-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Rat
• Immunogen: Recombinant human Cytokeratin 4 protein (350-480aa)
• Conjugation: Unconjugated
• Alternative Names: CK 4, CK4, CYK 4, CK-4, CYK4, Cytokeratin4, Cytokeratin-4, FLJ31692, K4, Keratin 4, Keratin-4, Keratin type II cytoskeletal 4, Keratin4, KRT 4, KRT4, K2C4_HUMAN, Keratin, type II cytoskeletal 4, K4, Type-II keratin Kb4.

Product Description

Cytokeratin 4 Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [2F9]
• Molecular Weight: 57 kDa
• UniProt: P19013
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: KRT4

Application Notes

• Application Dilute: WB=1:500-2000

Images

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 4) Monoclonal Antibody, Unconjugated (orb559075) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 4) Monoclonal Antibody, Unconjugated (orb559075) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 4) Monoclonal Antibody, Unconjugated (orb559075) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat esophageal), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 4) Monoclonal Antibody, Unconjugated (orb559075) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat tongue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Cytokeratin 4) Monoclonal Antibody, Unconjugated (orb559075) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Rat Esophagus Lysates, Lane 2: Rat Tongue Lysates, Primary: Anti-Cytokeratin 4 (orb559075) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57kDa, Observed band size: 57kDa.

IHC-P analysis of Human esophageal using Cytokeratin 4 Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of mouse esophageal using Cytokeratin 4 Monoclonal Antibody at 1:200 dilution.