Histone H3.3 Recombinant Rabbit Monoclonal Antibody, Clone: [7B12], Unconjugated

Product Overview

• Article Name: Histone H3.3 Recombinant Rabbit Monoclonal Antibody, Clone: [7B12], Unconjugated
• Biozol Catalog Number: BYT-ORB559096
• Supplier Catalog Number: orb559096
• Alternative Catalog Number: BYT-ORB559096-100,BYT-ORB559096-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant human Histone H3.3 protein, full length.
• Conjugation: Unconjugated
• Alternative Names: H3 histone family 3A, H3 histone family 3B, H3 histone, family 3B(H3.3B), H3.3, H3.3A, H3.3B, H33_HUMAN, H3F3, H3F3A, H3f3b, Histone H3.3Q, Histone H3.A, Histone H3.B, MGC87782, MGC87783.

Product Description

Histone H3.3 Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [7B12]
• Molecular Weight: 15 kDa
• UniProt: P84243
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: H3-3A

Application Notes

• Application Dilute: WB=1:200-1000, IHC-P=1:100-400, IHC-F=1:100-400, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000

Images

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H3.3) Monoclonal Antibody, Unconjugated (orb559096) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human SY5Y cell lysates, Lane 2: Human MOLT-4 cell lysates, Lane 3: Human THP-1 cell lysates, Lane 4: Human 293T Cell Lysates, Lane 5: Human K562 Cell Lysates, Lane 6: Human U251 Cell Lysates, Lane 7: Human HELA Cell Lysates, Primary: Anti-Histone H3.3 (15kD) (orb559096) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kDa, Observed band size: 15 kDa.

IHC-P analysis of human skin cancer using Histone H3.3 Monoclonal Antibody at 1:200 dilution.