Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody, Clone: [7A12], Unconjugated

Product Overview

• Article Name: Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody, Clone: [7A12], Unconjugated
• Biozol Catalog Number: BYT-ORB559128
• Supplier Catalog Number: orb559128
• Alternative Catalog Number: BYT-ORB559128-100,BYT-ORB559128-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human RPS6KA1 around the phosphorylation site of Ser380 GF(p-S)FV
• Conjugation: Unconjugated
• Alternative Names: RSK1(S380), RPS6KA1(phospho Ser380), RPS6KA1(phospho S380), KS6A1_HUMAN, Ribosomal protein S6 kinase alpha-1, EC:2.7.11.1, MAPKAPK1A, RSK1, S6K-alpha-1, 90 kDa ribosomal protein S6 kinase 1(p90-RSK 1, p90RSK1, p90S6K), MAP kinase-activated protein kinase 1a(MAPK-activated protein kinase 1a, MAPKAP kinase 1a, MAPKAPK-1a), Ribosomal S6 kinase 1(RSK-1), ribosomal protein S6 kinase A1, RSK, HU-1, p90Rsk, MAPKAPK1, RSK1 p90,

Product Description

Phospho-RPS6KA1 (Ser380) Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [7A12]
• Molecular Weight: 90 kDa
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: RPS6KA1

Application Notes

• Application Dilute: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

293T cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Phospho-RSK1 (S380)) monoclonal Antibody, Unconjugated (orb559128) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (orb559128) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-RPS6KA1 (Ser380)) Monoclonal Antibody, Unconjugated (orb559128) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: A431 cell lysates, Primary: Anti-Phospho-RPS6KA1 (Ser380) (orb559128) at 1/1000 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 81 kD, Observed band size: 81 kD.

IHC-P analysis of rat testis using Phospho-RPS6KA1 Ser380 Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of human colon using Phospho-RPS6KA1 Ser380 Monoclonal Antibody at 1:200 dilution.