Phospho-PKR (Thr446) Recombinant Rabbit Monoclonal Antibody, Clone: [10A1], Unconjugated

Product Overview

• Article Name: Phospho-PKR (Thr446) Recombinant Rabbit Monoclonal Antibody, Clone: [10A1], Unconjugated
• Biozol Catalog Number: BYT-ORB559134
• Supplier Catalog Number: orb559134
• Alternative Catalog Number: BYT-ORB559134-100,BYT-ORB559134-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: A synthesized peptide derived from human eIF2A K2 around the phosphorylation site of T446 KR-pT-RS
• Conjugation: Unconjugated
• Alternative Names: PKR(T446), PKR(phospho T446), p-PKR(phospho T446), PKR(phospho-Thr446), double-stranded RNA-dependent Protein Kinase, interferon-induced, double-stranded RNA-activated protein kinase isoform a, protein kinase, interferon-inducible double stranded RNA dependent, interferon-inducible elF2alpha kinase, double stranded RNA activated protein kinase, p68 kinase, eIF-2A protein kinase 2, P1/eIF-2A protein kinase, protein kinase RNA-activated, interferon-inducible RNA-dependent protein kinase, EIF2AK2, EIF2AK1, MGC126524, PKR, PRKRv, E2AK2_HUMAN.

Product Description

Phospho-PKR (Thr446) Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [10A1]
• Molecular Weight: 62 kDa
• UniProt: P19525
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: EIF2AK2

Application Notes

• Application Dilute: WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (orb559134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (orb559134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (orb559134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (orb559134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (orb559134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-PKR (Thr446)) Monoclonal Antibody, Unconjugated (orb559134) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Hela (Human) Cell Lysate at 30 ug, Lane 2: Hcclm3 (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-PKR (Thr446) (orb559134) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 67 kD.

IHC-P analysis of mouse placenta using Phospho-PKR Thr446 Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of rat brain using Phospho-PKR Thr446 Monoclonal Antibody at 1:200 dilution.