Villin Recombinant Rabbit Monoclonal Antibody, Clone: [6G2], Unconjugated

Product Overview

• Article Name: Villin Recombinant Rabbit Monoclonal Antibody, Clone: [6G2], Unconjugated
• Biozol Catalog Number: BYT-ORB559140
• Supplier Catalog Number: orb559140
• Alternative Catalog Number: BYT-ORB559140-100,BYT-ORB559140-25,BYT-ORB559140-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant human villin protein
• Conjugation: Unconjugated
• Alternative Names: VIL, VIL1, Villin 1, Villin-1, Villin1, D2S1471, OTTHUMP00000164145, VILI_HUMAN.

Product Description

Villin Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [6G2]
• Molecular Weight: 93 kDa
• UniProt: P09327
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: VIL1

Application Notes

• Application Dilute: WB=1:500-1000, IHC-P=1:20-200, IHC-F=1:20-200, IF=1:50-200

Images

Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (villin) Monoclonal Antibody, Unconjugated (orb559140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Villin) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (villin) Monoclonal Antibody, Unconjugated (orb559140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (villin) Monoclonal Antibody, Unconjugated (orb559140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (villin) Monoclonal Antibody, Unconjugated (orb559140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (villin) Monoclonal Antibody, Unconjugated (orb559140) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Colon tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Rat Kidney tissue lysates, Primary: Anti-Villin at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 93 kDa, Observed band size: 95 kDa.

Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-villin (orb559140) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 93 kD, Observed band size: 93 kD.