C-Kit Recombinant Rabbit Monoclonal Antibody, Clone: [7C8], Unconjugated

Product Overview

• Article Name: C-Kit Recombinant Rabbit Monoclonal Antibody, Clone: [7C8], Unconjugated
• Biozol Catalog Number: BYT-ORB559218
• Supplier Catalog Number: orb559218
• Alternative Catalog Number: BYT-ORB559218-25,BYT-ORB559218-50,BYT-ORB559218-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: Recombinant human c-Kit protein, around C-terminal 200aa
• Conjugation: Unconjugated
• Alternative Names: C-Kit, CD117, MASTC, PBT, SCFR, Bs, Fdc, Gsfsco1, Gsfsco5, Gsfsow3, SCO1, SCO5, SOW3, Ssm, Tr-kit, W, KIT_HUMAN, KIT, Piebald trait protein (PBT), Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, 2.7.10.1, KIT_MOUSE, Sl,

Product Description

C-Kit Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [7C8]
• Molecular Weight: 105 kDa
• UniProt: P10721
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: KIT

Application Notes

• Application Dilute: WB=1:1000-2000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000

Images

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse ovary), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (c-Kit) Monoclonal Antibody, Unconjugated (orb559218) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P analysis of human breast carcinoma using c-Kit Monoclonal Antibody at 1:50 dilution.

IHC-P analysis of mouse ovary using c-Kit Monoclonal Antibody at 1:200 dilution.