LAMP2 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: LAMP2 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB5609
• Supplier Catalog Number: orb5609
• Alternative Catalog Number: BYT-ORB5609-100,BYT-ORB5609-200,BYT-ORB5609-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human LAMP2 (301-411/411aa)
• Conjugation: Unconjugated
• Alternative Names: CD107 antigen-like family member B, CD107b, CD107b antigen, Igp110, Igp2, LAMP 2, LAMP 2C, LAMP B, LAMP-2, LAMP2_RAT, LAMPB, LGP110, Lysosomal associated membrane protein 2C, Lysosome associated membrane glycoprotein 2 precursor, Lysosome associated membrane protein 2, Lysosome-associated membrane glycoprotein 2, Lysosome-associated membrane protein 2, MAC3.

Product Description

LAMP2 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 120 kDa
• Pubmed: 29184609
• UniProt: P13473
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: LAMP2

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1ug/Test, IP=1:20-50

Images

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (orb5609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (orb5609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (orb5609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (orb5609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (LAMP2) Polyclonal Antibody, Unconjugated (orb5609) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: U251 (Human) Cell Lysate at 30 ug, Lane 2: HepG2 (Human) Cell Lysate at 30 ug, Lane 3: 293T (Human) Cell Lysate at 30 ug, Lane 4: HL-60 (Human) Cell Lysate at 30 ug, Lane 5: HUVEC (Human) Cell Lysate at 30 ug, Lane 6: RAW264.7 (Mouse) Cell Lysate at 30 ug, Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug, Primary: Anti-LAMP2 (orb5609) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 120 kD.

Tissue/Cell: mouse lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-LAMP2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-LAMP2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.