ATG5/APG5L Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: ATG5/APG5L Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB5704
• Supplier Catalog Number: orb5704
• Alternative Catalog Number: BYT-ORB5704-100,BYT-ORB5704-200,BYT-ORB5704-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human APG5L (101-200/275aa)
• Conjugation: Unconjugated
• Alternative Names: ATG5, APG 5, APG 5L, APG5, APG5 autophagy 5 like, APG5 like, APG5-like, APG5L, Apoptosis specific protein, Apoptosis-specific protein, ASP, ATG 5, ATG5, ATG5 autophagy related 5 homolog, Autophagy protein 5, hAPG5, Homolog of S Cerevisiae autophagy 5, ATG5_HUMAN.

Product Description

ATG5/APG5L Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 32 kDa
• Pubmed: 28130038
• UniProt: Q9H1Y0
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: ATG5

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

Images

Blank control: A431. Primary Antibody (green line): Rabbit Anti-ATG5 antibody (orb5704), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (orb5704) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (orb5704) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATG5) Polyclonal Antibody, Unconjugated (orb5704) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATG5/APG5L) Polyclonal Antibody, Unconjugated (orb5704) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Kidney tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Primary: Anti-ATG5/APG5 (orb5704) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kDa, Observed band size: 47 kDa.

IHC-P analysis of human gastric using ATG5 Polyclonal Antibody at 1:200 dilution.

IHC-P analysis of Mouse brain using ATG5 Polyclonal Antibody at 1:400 dilution.