HEY1 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: HEY1 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB574241
• Supplier Catalog Number: orb574241
• Alternative Catalog Number: BYT-ORB574241-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human HEY1
• Conjugation: Unconjugated
• Alternative Names: CHF2, OAF1, HERP2, HESR1, HRT-1, NERP2, hHRT1, BHLHb31

Product Description

Rabbit polyclonal antibody to HEY1

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 33 kDa
• NCBI: 036390
• UniProt: Q9Y5J3
• Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: PQNGHGNAGTTASPTEPHHQGRLGSAHPEAPALRAPPSGSFGPVLPVVTS
• Target: HEY1

Images

Sample Tissue: Human 293T Whole Cell, Antibody dilution: 1 ug/ml.

Sample Tissue: Human 293T, Antibody dilution: 1.0 ug/ml.

Sample Tissue: Human ACHN Whole Cell, Antibody dilution: 3 ug/ml.

Sample Type: Hela Whole Cell lysates, Antibody dilution: 1.0 ug/ml.

Positive control (+): NCI-H226 (N13), Negative control (-): U937 (N31), Antibody concentration: 1 ug/ml.

Rabbit Anti-HEY1 Antibody, Paraffin Embedded Tissue: Human Kidney, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

Rabbit Anti-HEY1 Antibody, Paraffin Embedded Tissue: Human Lung, Cellular Data: Alveolar cells, Antibody Concentration: 4.0-8.0 ug/ml, Magnification: 400X.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-HEY1 Antibody, Titration: 2.5 ug/ml, Positive Control: Lung.