Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB5768
• Supplier Catalog Number: orb5768
• Alternative Catalog Number: BYT-ORB5768-100,BYT-ORB5768-200,BYT-ORB5768-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Ser33/37 LD(p-S)GIH(p-S)GA
• Conjugation: Unconjugated
• Alternative Names: CTNB1_HUMAN, Catenin beta-1, catenin beta 1, CTNNB1, Catenin beta, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286, EVR7, MRD19, NEDSDV, armadillo, betacatenin, beta catenin, beta-catenin,

Product Description

Phospho-Beta-Catenin (Ser33 + Ser37) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 86 kDa
• UniProt: P35222
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: CTNNB1

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2µg /test
• Application Notes: Modification: Phosphorylated

Images

Immunohistochemical staining of rat brain tissue using beta Catenin (phospho-Ser33-37) antibody.

Immunohistochemical staining of rat brain tissue using beta Catenin (phospho-Ser33-37) antibody.

Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4C) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Beta-Catenin (Ser33+37) antibody (orb5768), dilution: 0.2 µg/10 6 cells. Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse intestine tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Beta-Catenin (Ser33 + Ser37)) Polyclonal Antibody, Unconjugated (orb5768) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-Beta-Catenin (Ser33+37) Polyclonal Antibody, Unconjugated (orb5768) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-Beta-Catenin (Ser33+37) Polyclonal Antibody, Unconjugated (orb5768) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.