CHAC1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB578544
Article Name: CHAC1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB578544
Supplier Catalog Number: orb578544
Alternative Catalog Number: BYT-ORB578544-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: WB
Species Reactivity: Human
Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CHAC1
Conjugation: Unconjugated
Alternative Names: MGC4504
Rabbit polyclonal antibody to CHAC1
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 24 kDa
NCBI: 077016
UniProt: Q9BUX1
Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence: Synthetic peptide located within the following region: TPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQD
Target: CHAC1
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. Peptide is present in 24 kDa canonical isoform as well as 29 kDa isoform and a 19 kDa isoform. Higher molecular weight bands may indicate enzymatic intermediates involving the full length protein.
Sample Tissue: Human Du145 Whole Cell, Antibody Dilution: 5 ug/ml.
Sample Tissue: Human H69 Whole Cell, Antibody Dilution: 5 ug/ml.
Sample Tissue: Human RPMI 8226 Whole Cell, Antibody Dilution: 1 ug/ml.
Positive control (+): MCF7 (N10), Negative control (-): Human liver (LI), Antibody concentration: 0.5 ug/ml.
WB Suggested Anti-CHAC1 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: 721_B cell lysate. CHAC1 is supported by BioGPS gene expression data to be expressed in 721_B.