SIAH2 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: SIAH2 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB583856
• Supplier Catalog Number: orb583856
• Alternative Catalog Number: BYT-ORB583856-100
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SIAH2
• Conjugation: Unconjugated
• Alternative Names: hSiah2

Product Description

Rabbit polyclonal antibody to SIAH2

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Molecular Weight: 35 kDa
• NCBI: 005058
• UniProt: O43255
• Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Sequence: Synthetic peptide located within the following region: SRPSSTGPSANKPCSKQPPPQPQHTPSPAAPPAAATISAAGPGSSAVPAA
• Target: SIAH2

Images

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment.

Sample Tissue: Human OVCAR-3 Whole Cell, Antibody dilution: 3 ug/ml.

Sample Type: Hela, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1 ug/ml, Peptide Concentration: 5 ug/ml, Lysate Quantity: 25 ug/lane/lane, Gel Concentration: 0.12.

Sample Type: HepG2, Antibody dilution: 1.0 ug/ml. SIAH2 is supported by BioGPS gene expression data to be expressed in HepG2.

Sample Type: MCF7, Antibody dilution: 1.0 ug/ml. SIAH2 is supported by BioGPS gene expression data to be expressed in MCF7.

Lanes: 1: 50 ug HEK293T lysate, 2: 50 ug A549 lysate, Primary Antibody dilution: 1:1000, Secondary Antibody: Anti-rabbit-HRP, Secondary Antibody dilution: 1:5000, Gene Name: SIAH2.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/ml. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:1562500, Positive Control: Hela cell lysate.