S100 beta Recombinant Rabbit Monoclonal Antibody, Clone: [26C3], Unconjugated

Product Overview

• Article Name: S100 beta Recombinant Rabbit Monoclonal Antibody, Clone: [26C3], Unconjugated
• Biozol Catalog Number: BYT-ORB612224
• Supplier Catalog Number: orb612224
• Alternative Catalog Number: BYT-ORB612224-100,BYT-ORB612224-25,BYT-ORB612224-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: A synthesized peptide derived from human S100B (50-92aa)
• Conjugation: Unconjugated
• Alternative Names: NEF, Protein S100 B, Protein S100-B, S 100 calcium binding protein beta chain, S 100 protein beta chain, S 100 protein beta subunit, S-100 protein beta chain, S-100 protein subunit beta, S100, S100 calcium binding protein B, S100 calcium binding protein beta (neural), S100 calcium binding protein beta chain, S100 calcium-binding protein B, S100 protein beta chain, S100B_HUMAN, S100beta.

Product Description

S100 beta Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [26C3]
• Molecular Weight: 10 kDa
• UniProt: P04271
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: S100B

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100, ICC/IF=1:50-200

Images

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100 beta) Monoclonal Antibody, Unconjugated (orb612224) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100 beta) Monoclonal Antibody, Unconjugated (orb612224) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100 beta) Monoclonal Antibody, Unconjugated (orb612224) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100 beta) Monoclonal Antibody, Unconjugated (orb612224) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Human SH-SY5Y cell lysates, Primary: Anti-S100 beta (orb612224) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 10 kDa, Observed band size: 10 kDa.

Sample: Lane 1: mouse liver tissue lysates, Primary: Anti-S100 beta (orb612224) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 10 kD, Observed band size: 13 kD.

Sample: Lane 1: zebrafish tissue tissue lysates, Primary: Anti-S100 beta (orb612224) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 10 kD, Observed band size: 13 kD.

IHC-P analysis of mouse brain using S100 beta Monoclonal Antibody at 1:50 dilution.

IHC-P analysis of rat brain using S100 beta Monoclonal Antibody at 1:200 dilution.