Thyroid Hormone Receptor Antibody (Biotin), IgG2a, Clone: [H43], Mouse, Monoclonal

Product Overview

• Article Name: Thyroid Hormone Receptor Antibody (Biotin), IgG2a, Clone: [H43], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB612668
• Supplier Catalog Number: orb612668
• Alternative Catalog Number: BYT-ORB612668-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide from the full length Human Thyroid hormone receptor protein
• Conjugation: Biotin
• Alternative Names: ERBA ALPHA, ERBA beta, ERBA1, ERBA2, NR1A1, NR1A2, THR1, THRA, THRA1, THRA2, THRB, THRB1, THRB2, Thyroid hormone receptor alpha, Thyroid hormone receptor beta

Product Description

Mouse monoclonal antibody against NR1D1 conjugated to Biotin

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [H43]
• Molecular Weight: 70 kDa
• Isotype: IgG2a
• NCBI: 001190918
• UniProt: P10827
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: Thyroid Hormone Receptor

Application Notes

• Application Dilute: WB (1:500), IHC (1:100)
• Application Notes: Application Notes: A 1:500 dilution of SMC-561 was sufficient for detection of Pan-Thyroid hormone receptor in 10 ug of Hep G2 Human Hepatoblastoma Cell lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 7.5X.

Immunohistochemistry analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 4X.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Thyroid Hormone Receptor Antibody. (D) Hoechst (E) Composite.

Western Blot analysis of Human Hep G2 Hepatoblastoma Cell lysate showing detection of Thyroid Hormone Receptor protein using Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody, Clone H43. Load: 10 ug. Primary Antibody: Mouse Anti-Thyroid Hormone Receptor Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Other Band (s): Higher molceular weight bands could be due to PTMs.