parvalbumin Antibody (Biotin), IgG2a, Clone: [C12], Mouse, Monoclonal

Product Overview

• Article Name: parvalbumin Antibody (Biotin), IgG2a, Clone: [C12], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB612706
• Supplier Catalog Number: orb612706
• Alternative Catalog Number: BYT-ORB612706-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide from the full length Rat Parvalbumin protein
• Conjugation: Biotin
• Alternative Names: Parvalbumin alpha, PRVA_HUMAN, PVALB

Product Description

Mouse monoclonal antibody against Paravalbumin conjugated to Biotin

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [C12]
• Molecular Weight: 12 kDa
• Isotype: IgG2a
• NCBI: 071944
• UniProt: P02625
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: parvalbumin

Application Notes

• Application Dilute: WB (1:500), IHC (1:100)
• Application Notes: Application Notes: A 1:500 dilution of SMC-563 was sufficient for detection of Parvalbumin in 15 ug of Rat Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid Cancer. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Immunohistochemistry analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Thyroid. Species: Mouse. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:100 for Overnight at 4C, then 30 min at 37C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37C. Counterstain: DAPI for 3 min at RT. Magnification: 5X.

Western Blot analysis of Rat brain showing detection of Parvalbumin protein using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Load: 15 ug. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Parvalbumin Monoclonal Antibody, Clone C12. Tissue: Undifferentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-Parvalbumin Monoclonal Antibody at 1:250. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) Parvalbumin Antibody. (D) Hoechst (E) Composite.