Tau Antibody (Biotin), IgG1, Clone: [3D4], Mouse, Monoclonal

Product Overview

• Article Name: Tau Antibody (Biotin), IgG1, Clone: [3D4], Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB612877
• Supplier Catalog Number: orb612877
• Alternative Catalog Number: BYT-ORB612877-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: DOT, ELISA, ICC, IF, WB
• Species Reactivity: Human, Mouse
• Immunogen: Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils
• Conjugation: Biotin
• Alternative Names: Tau, TAU, TAU_HUMAN, MAPT, MAPTL, Microtubule-associated protein tau, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, Neurofibrillary tangle protein, Paired helical filament tau, Paired helical filament-tau, PHF tau, PHF-tau, DDPAC, FTDP 17, G protein beta1/gamma2 subunit interacting factor 1, MSTD, Mtapt, MTBT1, MTBT2, PPND, PPP1R103, Protein phosphatase 1 regulatory subunit 103, RNPTAU, AI413597, AW045860, FLJ31424, MGC134287, MGC138549, MGC156664

Product Description

Mouse monoclonal antibody against Tau conjugated to Biotin

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/mL
• Clone Designation: [3D4]
• Isotype: IgG1
• NCBI: 005901
• UniProt: P10636
• Buffer: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
• Target: Tau

Application Notes

• Application Dilute: WB (1:1000)
• Application Notes: Application Notes: A 1:1000 dilution of SMC-608 was sufficient for detection of Tau 2N4R P301S Fibril in 20 ug of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody

Images

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4. Tissue: iPSC-derived neurons. Species: Human. Fixation: 4% PFA. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:100 for Overnight at 4C. Counterstain: DAPI at 1:5000 for 5 minutes at RT in the dark. Magnification: 40X.

Western Blot analysis of Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4. Lane 1: MW Marker. Lane 2: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4. Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.