Human Mesothelin protein

Product Overview

• Article Name: Human Mesothelin protein
• Biozol Catalog Number: BYT-ORB624114
• Supplier Catalog Number: orb624114
• Alternative Catalog Number: BYT-ORB624114-1,BYT-ORB624114-100,BYT-ORB624114-20
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: CAK1 antigen (Pre-pro-megakaryocyte-potentiating factor) (Megakaryocyte-potentiating factor) (Mesothelin, cleaved form) (MPF)

Product Description

This Human Mesothelin protein spans the amino acid sequence from region 296-580aa. Purity: Greater than 92% as determined by SDS-PAGE.

Product Properties

• Molecular Weight: 59.6 kDa
• UniProt: Q13421
• Buffer: Lyophilized from a 0.2 µm sterile filtered PBS, 6% Trehalose, pH 7.4
• Source: Homo sapiens (Human)
• Purity: Greater than 92% as determined by SDS-PAGE.
• Form: Lyophilized powder
• Sequence: EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGL

Application Notes

• Application Notes: Biological Origin: Homo sapiens (Human). Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized MSLN at 2 µg/ml can bind Anti-MSLN rabbit monoclonal antibody, the EC50 of the MSLN protein is 2.657-3.177 ng/ml. Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized MSLN at 2 µg/ml can bind Anti-MSLN rabbit monoclonal antibody, the EC50 of the MSLN protein is 2.657-3.177 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized MUC16 at 10 µg/ml can bind MSLN, the EC50 is 460.7-662.2 ng/ml.