Recombinant Human Tumor necrosis factor (TNF) Protein

Product Overview

• Article Name: Recombinant Human Tumor necrosis factor (TNF) Protein
• Biozol Catalog Number: BYT-ORB624131
• Supplier Catalog Number: orb624131
• Alternative Catalog Number: BYT-ORB624131-1,BYT-ORB624131-100,BYT-ORB624131-20
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: Cachectin TNF-alpha Tumor necrosis factor ligand superfamily member 2

Product Description

This Recombinant Human Tumor necrosis factor (TNF) Protein spans the amino acid sequence from region 77-233aa. Purity: Greater than 90% as determined by SDS-PAGE.

Product Properties

• Molecular Weight: 19.4 kDa
• UniProt: P01375
• Buffer: Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0, Tris-based buffer,50% glycerol
• Source: Homo sapiens (Human)
• Purity: Greater than 90% as determined by SDS-PAGE.
• Form: Liquid or Lyophilized powder
• Sequence: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Application Notes

• Application Notes: Biological Origin: Homo sapiens (Human). Biological Activity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 33.32-47.38 pg/mL. Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference

Images

orb624131

Measured by its binding ability in a functional ELISA. Immobilized Human TNF at 5 µg/ml can bind Human TNFR2 protein. The EC50 is 3.470-4.107 ng/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.