Phospho-Lyn (Tyr397) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-Lyn (Tyr397) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB6340
• Supplier Catalog Number: orb6340
• Alternative Catalog Number: BYT-ORB6340-100,BYT-ORB6340-200,BYT-ORB6340-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human Lyn around the phosphorylation site of Tyr397 NE(p-Y)TA
• Conjugation: Unconjugated
• Alternative Names: ALyn(phospho Y397), Lyn(phospho Tyr397), p-Lyn(Tyr397), Hck 2, JTK 8, JTK8, ONCOGENE LYN, Tyrosine protein kinase LYN, V yes 1 Yamaguchi sarcoma viral related oncogene homolog, Yamaguchi sarcoma viral(v yes 1) related oncogene homolog, AA407514, EC 2.7.10.2, FLJ26625, LYN_HUMAN.

Product Description

Phospho-Lyn (Tyr397) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 56 kDa
• UniProt: P07948
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: LYN

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (mouse lung tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (orb6340) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse spleen tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (orb6340) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat embryo), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (orb6340) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-Lyn (Tyr397)) Polyclonal Antibody, Unconjugated (orb6340) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Embryo tissue lysates, Lane 3: Mouse BV2 cell lysates, Lane 4: Mouse Raw264.7 cell lysates, Lane 5: Human A431 cell lysates, Lane 6: Human Raji cell lysates, Primary: Anti-Phospho-Lyn (Tyr397) (orb6340) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 58 kDa.

Sample: Placenta (Mouse) Lysate at 40 ug, Lung (Mouse) Lysate at 40 ug, Primary: Anti-Phospho-Lyn (Tyr397) (orb6340) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56kD, Observed band size: 63kD.

Western blot analysis of mouse placenta and lung using Lyn (phospho-Tyr397) antibody.

IHC-P image of mouse spleen tissue using Lyn (phospho-Tyr397) antibody

IHC-P image of mouse lung tissue using Lyn (phospho-Tyr397) antibody