Biotin-11-dUTP

Product Overview

• Article Name: Biotin-11-dUTP
• Biozol Catalog Number: BYT-ORB64042
• Supplier Catalog Number: orb64042
• Alternative Catalog Number: BYT-ORB64042-5
• Manufacturer: Biorbyt
• Category: Biochemikalien

Product Description

Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/50% dTTP.

Product Properties

• Concentration: 1.0 mM-1.1 mM
• Molecular Weight: Theoretical MW: 862.67 g/mol (free acid), Detected MW: 862.18 g/mol (free acid)
• Purity: 95% (HPLC)
• Form: filtered solution (30 kDa) in 10 mM Tris-HCl, Color: colorless to slightly yellow, pH: 7.5 0.5
• Formula: C28H45N6O17P3S

Application Notes

• Application Notes: Application Notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data, Nick Translation with DNAse I/DNA Polymerase I & in-house data, Primer Extension with Klenow 3-5 exo-, 3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT), Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: lambdamax 240/289 nm, epsilon 10.7/7.1 L mmol-1 cm-1, (Tris-HCl pH 7.5)

Images

Chemical structure of Biotin-11-dUTP (Biotin).