Phospho-MDM2 (Thr218) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-MDM2 (Thr218) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB6405
• Supplier Catalog Number: orb6405
• Alternative Catalog Number: BYT-ORB6405-100,BYT-ORB6405-200,BYT-ORB6405-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human MDM2 around the phosphorylation site of Thr218 TG(p-T)P-NH2
• Conjugation: Unconjugated
• Alternative Names: MDM2(phospho T218), MDM2(phospho Thr218), p-MDM2(T218), p-MDM2(Thr218), Double minute 2 protein, Hdm 2, HDM2, MDM 2, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein(mouse) binding protein 104kDa, MDM2BP, Mouse Double Minute 2, MTBP, Murine Double Minute Chromosome 2, Oncoprotein Mdm2, p53 Binding Protein Mdm2, Ubiquitin protein ligase E3 Mdm2, MDM2_HUMAN, E3 ubiquitin-protein ligase Mdm2.

Product Description

Phospho-MDM2 (Thr218) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 55 kDa
• UniProt: Q00987
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: MDM2

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Application Notes: Modification: Phosphorylated

Images

Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-Phospho-MDM2 (Thr218) antibody (orb6405), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-MDM2 (Thr218)) Polyclonal Antibody, Unconjugated (orb6405) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-MDM2 (Thr218)) Polyclonal Antibody, Unconjugated (orb6405) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-MDM2 (Thr218)) Polyclonal Antibody, Unconjugated (orb6405) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-MDM2 (Thr218)) Polyclonal Antibody, Unconjugated (orb6405) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-MDM2 (Thr218)) Polyclonal Antibody, Unconjugated (orb6405) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human gastric carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-MDM2 (Thr218) Polyclonal Antibody, Unconjugated (orb6405) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-Phospho-MDM2 (Thr218) Polyclonal Antibody, Unconjugated (orb6405) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate bu