Biotin-16-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of uridine. Recommended Biotin-16-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-16-dUTP/50% dTTP.
filtered solution (30 kDa) in water, Color: colorless to slightly yellow, pH: 7.5 0.5
Formula:
C32H52N7O18P3S
Application Notes:
Application Notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data, Nick Translation with DNAse I/DNA Polymerase I & in-house data, Primer Extension with Klenow exo-, 3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT), Reverse Transcription with MMLV Reverse Transcriptase. Incorporation into RNA by 3-End Labeling with Terminal deoxynucleotidyl Transferase (TdT). Spectroscopic Propertie: lambdamax 240 nm, epsilon 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5)
Structural formula of Biotin-16-dUTP
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