Human Novel Coronavirus Spike glycoprotein(S) protein

Product Overview

• Article Name: Human Novel Coronavirus Spike glycoprotein(S) protein
• Biozol Catalog Number: BYT-ORB668860
• Supplier Catalog Number: orb668860
• Alternative Catalog Number: BYT-ORB668860-20,BYT-ORB668860-100,BYT-ORB668860-1
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: /

Product Description

This Human Novel Coronavirus Spike glycoprotein(S) protein spans the amino acid sequence from region 319-541aa. Purity: Greater than 90% as determined by SDS-PAGE.

Product Properties

• Molecular Weight: 30.1 kDa
• UniProt: P0DTC2
• Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Source: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
• Purity: Greater than 90% as determined by SDS-PAGE.
• Form: Lyophilized powder
• Sequence: RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF

Application Notes

• Application Notes: Biological Origin: Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2). Application Notes: SARS-CoV-2 Related Proteins

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 µg/ml can bind SARS-CoV-2-S Antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 27.96 - 33.35 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 µg/ml can bind SARS-CoV-2-S Antibody, the EC50 of SARS-CoV-2-S1-RBD protein is 13.96 -16.62 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 5 µg/ml can bind human ACE2, the EC50 is 2.785-9.139 ng/ml.

SARS-CoV-2 Spike protein RBD his/myc tag captured on COOH chip can bind Human ACE2 protein Fc tag with an affinity constant of 13.8 nM as detected by LSPR Assay.

Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 µg/ml can bind Biotinylated human ACE2, the EC50 is 4.599-8.322 ng/ml