Phospho-PKC delta (Tyr311) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-PKC delta (Tyr311) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB6751
• Supplier Catalog Number: orb6751
• Alternative Catalog Number: BYT-ORB6751-100,BYT-ORB6751-200,BYT-ORB6751-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human PKC delta around the phosphorylation site of Tyr311 GI(p-Y)QG
• Conjugation: Unconjugated
• Alternative Names: PKC delta(phospho Y311), p-PKC delta(phospho Y311), MAY 1, MAY1, nPKC delta, PCKd, PKC d, PKC delta, PKC-d, PKCD, PKCdelta, PRKC D, PRKC delta, PRKC-d, PRKCd, Protein Kinase C delta, Protein kinase C delta type, Protein Kinase Cdelta, KPCD_HUMAN, PKC delta.

Product Description

Phospho-PKC delta (Tyr311) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 77 kDa
• UniProt: Q05655
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: PRKCD

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
• Application Notes: Modification: Phosphorylated

Images

Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-phospho-PKC delta (Tyr311) antibody (orb6751), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min) and then permeabilized with 90% ice-cold methanol for 20 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (blue line): A431 (Black). Primary Antibody (green line): Rabbit Anti-phospho-PKC delta (Tyr311) antibody (orb6751), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 20 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (mouse lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6751) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6751) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6751) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6751) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-PKC delta (Tyr52)) Polyclonal Antibody, Unconjugated (orb6751) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen

IHC-P analysis of mouse lung using phospho-PKC delta Tyr52 Polyclonal Antibody at 1:200 dilution.