Phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB6780
• Supplier Catalog Number: orb6780
• Alternative Catalog Number: BYT-ORB6780-100,BYT-ORB6780-200,BYT-ORB6780-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AKT1 around the phosphorylation site of Thr308 MK(p-T)FC
• Conjugation: Unconjugated
• Alternative Names: AKT1(phospho T308), AKT2(phospho T309), AKT3(phospho T305), p-AKT1(phospho T308), p-AKT2(phospho T309), p-AKT3(phospho T305), AKT 1, AKT, AKT1, AKT-1, AKT1_HUMAN, AKT 2, AKT2, AKT-2, AKT2_HUMAN, AKT 3, AKT3, AKT-3, AKT3_HUMAN, C AKT, cAKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB-ALPHA, PKB beta, PKB gamma, PRKBA, Protein Kinase B alpha, Protein kinase B, Proto-oncogene c-Akt, RAC alpha, RAC alpha serine/threonine protein kinase, RAC, RAC PK alpha, Rac protein kinase alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1.

Product Description

Phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 53 kDa
• UniProt: P31749
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: AKT1

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
• Application Notes: Modification: Phosphorylated

Images

Flow cytometric analysis of 4T1 Cell using AKT1 (phospho-Thr308-Thr309) antibody.

Flow cytometric analysis of EC9706 Cell using AKT1 (phospho-Thr308-Thr309) antibody.

Blank control (blue): EC9706 (fixed with 2% paraformaldehyde for 10 min at 37C). Primary Antibody: Rabbit Anti-phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) antibody (orb6780, Green), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305)) Polyclonal Antibody, Unconjugated (orb6780) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305)) Polyclonal Antibody, Unconjugated (orb6780) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.Antibody, Unconjugated (orb6780) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) (orb6780) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 62/53 kD, Observed band size: 62/53 kD.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305) (orb6780) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Adrenal gland (Mouse) Lysate at 40 ug, MCF-7 (Human) Cell Lysate at 30 ug, A549 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AKT1+AKT2+AKT3 (Thr308+Thr309+Thr305) (orb6780) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 60 kD.

Tissue/Cell: A549 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (phospho-AKT1 + AKT2 + AKT3 (Thr308+Thr309+Thr305)) polyclonal Antibody, Unconjugated (orb6780) 1:100, 90 minutes at 37C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.