Phospho-AKT3 (Ser472) Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-AKT3 (Ser472) Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB6790
• Supplier Catalog Number: orb6790
• Alternative Catalog Number: BYT-ORB6790-100,BYT-ORB6790-200,BYT-ORB6790-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human AKT3 around the phosphorylation site of Ser472 QF(p-S)YS
• Conjugation: Unconjugated
• Alternative Names: Akt3, AKT3 kinase, AKT3_HUMAN, DKFZp434N0250, PKB gamma, PKBG, PRKBG, Protein kinase Akt-3, Protein Kinase AKT3, Protein kinase B gamma, RAC gamma, RAC gamma serine/threonine protein kinase, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, RACPK gamma, Serine threonine protein kinase Akt 3, Serine threonine protein kinase Akt3, STK 2, STK-2, STK2, V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma.

Product Description

Phospho-AKT3 (Ser472) Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 56 kDa
• Pubmed: 38306274
• UniProt: Q9Y243
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: AKT3

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (orb6790) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (orb6790) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (orb6790) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-AKT3 (Ser472)) Polyclonal Antibody, Unconjugated (orb6790) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Huvec (Human) Cell Lysate at 30 ug, Lane 2: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-AKT3 (Ser472) (orb6790) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kD, Observed band size: 58 kD.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-AKT3 (Ser472) Polyclonal Antibody, Unconjugated (orb6790) 1:200, overnight at 4C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Western blot analysis of mouse lung lysate using AKT3 (phospho-Ser472) antibody

Western blot analysis of mouse brain lysate using AKT3 (phospho-Ser472) antibody

Western blot analysis of mouse lung lysate using AKT3 (phospho-Ser472) antibody