Human CD47 Protein, mFc-His Tag

Product Overview

• Article Name: Human CD47 Protein, mFc-His Tag
• Biozol Catalog Number: BYT-ORB689369
• Supplier Catalog Number: orb689369
• Alternative Catalog Number: BYT-ORB689369-10,BYT-ORB689369-50,BYT-ORB689369-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD47,MER6,IAP,OA3

Product Description

Recombinant human CD47 protein with C-terminal mouse Fc and 6*His tag

Product Properties

• Molecular Weight: The protein has a predicted molecular mass of 60 kDa after removal of the signal peptide.
• UniProt: Q08722
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Target: CD47

Images

Human CD47, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CD47, mFc-His tagged protein (orb689369) can bind its native ligand Human SIRPalpha, hFc-His tagged protein orb689370 in a linear range of 3.3-26.37 ng/ml.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CD47, mFc-His tagged protein (orb689369) can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) orb689306 in a linear range of 0.061-1.606 ng/ml.

ELISA plate pre-coated by 1 µg/ml (100 µL/well) Human CD47, mFc-His tagged protein (orb689369) can bind Anti-CD47 Neutralizing antibody orb1173650 in a linear range of 3.2-80.0 ng/ml.