Human SIRPa Protein, hFc-His Tag

Product Overview

• Article Name: Human SIRPa Protein, hFc-His Tag
• Biozol Catalog Number: BYT-ORB689370
• Supplier Catalog Number: orb689370
• Alternative Catalog Number: BYT-ORB689370-10,BYT-ORB689370-50,BYT-ORB689370-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS

Product Description

Recombinant Human SIRPa with C-terminal human Fc and 6*His tag

Product Properties

• Molecular Weight: The protein has a predicted molecular mass of 70-98 kDa after removal of the signal peptide.
• UniProt: P78324
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Target: SIRPa

Images

Human SIRPalpha, hFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CD47, mFc-His tagged protein orb689369 can bind its native ligand Human SIRPalpha, hFc-His tagged protein (orb689370) in a linear range of 3.3-26.37 ng/ml.

A: Human SIRPalpha, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRPalpha, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRPalpha, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRPalpha, hFc-His tagged protein after five freeze-thaw cycles.

Lane 1: Human SIRPalpha, hFc-His tagged protein without freeze-thaw treatment, Lane 2: Human SIRPalpha, hFc-His tagged protein after one freeze-thaw cycle, Lane 3: Human SIRPalpha, hFc-His tagged protein after three freeze-thaw cycles, Lane 4: Human SIRPalpha, hFc-His tagged protein after five freeze-thaw cycles.