Human PD-L1 Protein, mFc-His tag

Product Overview

• Article Name: Human PD-L1 Protein, mFc-His tag
• Biozol Catalog Number: BYT-ORB689384
• Supplier Catalog Number: orb689384
• Alternative Catalog Number: BYT-ORB689384-10,BYT-ORB689384-50,BYT-ORB689384-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1

Product Description

Recombinant human PD-L1 protein with C-terminal mouse Fc and 6*His tag

Product Properties

• Molecular Weight: The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
• UniProt: Q9NZQ7
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Target: PDL1

Images

Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human B7-1, hFc tagged protein orb689457 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 125-4000 ng/ml.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human B7-2, hFc tagged protein orb689458 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 62.5-4000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (orb689375) 1 µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.