Human Mesothelin (296-580) Protein, mFc-His Tag

Product Overview

• Article Name: Human Mesothelin (296-580) Protein, mFc-His Tag
• Biozol Catalog Number: BYT-ORB689392
• Supplier Catalog Number: orb689392
• Alternative Catalog Number: BYT-ORB689392-10,BYT-ORB689392-50,BYT-ORB689392-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: MSLN,Mesothelin,MPF

Product Description

Recombinant human Mesothelin(296-580) protein with C-terminal mouse Fc and 6*His tag

Product Properties

• Molecular Weight: The protein has a predicted molecular mass of 59.3 kDa after removal of the signal peptide.
• UniProt: Q13421
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Target: Mesothelin

Images

Human PD-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human PD1, mFc-His tagged protein (orb689386) can bind Anti-PD-1 Neutralizing antibody orb689311 in a linear range of 0.24-6.49 ng/ml.

Flow cytometry analysis with 2 µg/ml Human PD-1 Protein, mFc-His tag (orb689386) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2 µg/mL Human PD-1 Protein, mFc-His tag (orb689386) followed by PE-conjugated Goat anti-mouse IgG secondary antibody.