Human B7-2 Protein, mFc-His Tag

Product Overview

• Article Name: Human B7-2 Protein, mFc-His Tag
• Biozol Catalog Number: BYT-ORB689395
• Supplier Catalog Number: orb689395
• Alternative Catalog Number: BYT-ORB689395-10,BYT-ORB689395-50,BYT-ORB689395-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: CD86,B7-2,B70,CD28LG2,LAB72,MGC34413

Product Description

Recombinant human B7-2 protein with C-terminal mouse Fc and 6*His tag

Product Properties

• Molecular Weight: The protein has a predicted molecular mass of 52.4 kDa after removal of the signal peptide.
• UniProt: P42081
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Target: B7-2

Images

Human PD-L1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human PD-L1, mFc-His tagged protein orb689384 can bind Anti-PDL1 Neutralizing antibody orb689314 in a linear range of 0.24-7.81 ng/ml.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human PD1, hFc-His tagged protein orb689448 can bind Human PDL1, mFc-His tagged protein (orb689384) in a linear range of 62.5-251.1 ng/ml.

Flow cytometry analysis with 15 µg/mL Human PD-L1 Protein, mFc-His tag (orb689384) on Expi293 cells transfected with human PD1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).