Recombinant Human BAFF-R protein with C-terminal mouse Fc
Molecular Weight:
The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide.The apparent molecular mass of BAFF-R-mFc is approximately 35-55 kDa due to glycosylation.
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Purity:
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form:
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:
BAFF-R
Human IL6 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human IL6, mFc-His tagged protein (orb689393) can bind Anti-IL6 Neutralizing antibody orb689312 in a linear range of 7.81-386.8 ng/ml.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human IL6R, His tagged protein orb757423 can bind Human IL6, mFc-His tagged protein (orb689393) in a linear range of 0.512-40000 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human IL6R (A) were surface stained with Human IL6, mFc-His tagged protein (orb689393) 1 µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
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