Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Purity:
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form:
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:
DNAM1
Human PD-1 Protein, hFc-His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human PD1, hFc-His tagged protein (orb689448) can bind Human PDL1, mFc-His tagged protein orb689384 in a linear range of 62.5-251.1 ng/ml.
Flow cytometry analysis with 0.2 µg/ml Human PD-1 Protein, hFc-His tag (orb689448) on Expi293 cells transfected with human PD-L1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
HEK293 cell line transfected with irrelevant protein (red histogram) and human PD-L2 protein (blue histogram) were surface stained with 2 µg/mL Human PD-1 Protein, hFc-His tag (orb689448) followed by PE-conjugated Goat anti-human IgG secondary antibody.
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