Recombinant Human IL5 protein with C-terminal 6*His tag
Molecular Weight:
The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Purity:
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form:
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:
IL5
Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CD30, His tagged protein (orb689465) can bind Anti-CD30 Neutralizing antibody orb689322 in a linear range of 0.13-80.0 ng/ml.
ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CD30, His tagged protein (orb689465) can bind Human CD30 Ligand, mFc-His tagged protein orb689400 in a linear range of 2.56-320 ng/ml.
Flow cytometry analysis with 1 µg/mL Human CD30 Protein, His tag (orb689465) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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