Human TGFBR1 Protein, N-hFc Tag

Product Overview

• Article Name: Human TGFBR1 Protein, N-hFc Tag
• Biozol Catalog Number: BYT-ORB689441
• Supplier Catalog Number: orb689441
• Alternative Catalog Number: BYT-ORB689441-10,BYT-ORB689441-50,BYT-ORB689441-100
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Species Reactivity: Human
• Alternative Names: AAT5,ACVRLK4,ALK-5,ALK5,ESS1,LDS1,LDS1A,LDS2A,MSSE,SKR4,tbetaR-I,TBR-i,TBRI,TGFR-1

Product Description

Recombinant human TGFBR1 Protein with N-terminal Human Fc tag

Product Properties

• Molecular Weight: The protein has a predicted molecular mass of 36.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TGFBR1 is approximately 40-53 kDa due to glycosylation.
• UniProt: P36897
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Target: TGFBR1

Images

Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 µg/ml (100 µL/well) Human CTLA-4, hFc tagged protein (orb689463) can bind Anti-CTLA4 Neutralizing antibody orb689327 in a linear range of 0.64-80.0 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein (orb689463) 1 µg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Flow cytometry analysis with 1 µg/mL Human CTLA4 Protein, hFc tag (orb689463) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).