S100A4 Rabbit Polyclonal Antibody, Unconjugated

Product Overview

• Article Name: S100A4 Rabbit Polyclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB6910
• Supplier Catalog Number: orb6910
• Alternative Catalog Number: BYT-ORB6910-100,BYT-ORB6910-200,BYT-ORB6910-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, IF, IHC-Fr, IHC-P
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human S100A4 (15-101/101aa)
• Conjugation: Unconjugated
• Alternative Names: FSP1, FSP-1, FSP 1, 18A2, 42A, calcium Placental protein, Calcium protein, Calvasculin, CAPL, Fibroblast specific protein 1, FSP1, Leukemia multidrug resistance associated protein, Malignant transformation suppression 1, Metastasin, MTS1, Mts1 protein, Murine placental homolog, OTTHUMP00000015467, OTTHUMP00000015468, P9KA, PEL98, Placental calcium binding protein, Placental calcium-binding protein, Protein Mts1, Protein S100 A4, Protein S100-A4, S100 calcium binding protein A4(calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium binding protein A4, S100 calcium-binding protein A4, S100a4, S10A4_HUMAN

Product Description

S100A4 Rabbit Polyclonal Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1mg/ml
• Molecular Weight: 12 kDa
• UniProt: P26447
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: S100A4

Application Notes

• Application Dilute: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test

Images

Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-S100A4 antibody (orb6910), dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (orb6910) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (orb6910) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (S100A4) Polyclonal Antibody, Unconjugated (orb6910) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela Cell lysate at 30 ug, Primary: Anti-S100A4 (orb6910) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 12 kD, Observed band size: 15 kD.

Sample: Raji Cell lysate at 30 ug, Primary: Anti-S100A4 (orb6910) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000 dilution, Predicted band size: 12 kD, Observed band size: 12 kD.

Tissue/Cell: human lung cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-S100A4 Polyclonal Antibody, Unconjugated (orb6910) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining.

IHC-P analysis of Human breast carcinoma using S100A4 Polyclonal Antibody at 1:400 dilution.

IHC-P analysis of Rat bladder using S100A4 Polyclonal Antibody at 1:200 dilution.