Application Notes: Western blot, 0.25-0.5µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human Immunocytochemistry/Immunofluorescence, 5µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human ELISA, 0.1-0.5µg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500µg/ml
Flow Cytometry analysis of THP-1 cells using anti-NOX2/gp91phox/CYBB antibody. Overlay histogram showing THP-1 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NOX2/gp91phox/CYBB Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody. NOX2/gp91phox/CYBB was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-NOX2/gp91phox/CYBB Antibody overnight at 4C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody. NOX2/gp91phox/CYBB was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-NOX2/gp91phox/CYBB Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody. NOX2/gp91phox/CYBB was detected in paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-NOX2/gp91phox/CYBB Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody. NOX2/gp91phox/CYBB was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-NOX2/gp91phox/CYBB Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody. NOX2/gp91phox/CYBB was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-NOX2/gp91phox/CYBB Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody. NOX2/gp91phox/CYBB was detected in paraffin-embedded section of human testicular cancer tissue. Heat media
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