Filamin A/FLNA Antibody (monoclonal, 3F8), Clone: [3F8], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: Filamin A/FLNA Antibody (monoclonal, 3F8), Clone: [3F8], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: BYT-ORB692222
• Supplier Catalog Number: orb692222
• Alternative Catalog Number: BYT-ORB692222-100
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: IF, IHC, WB
• Species Reactivity: Human
• Immunogen: E.coli-derived human Filamin A/FLNA recombinant protein (Position: E451-Q638).
• Conjugation: Unconjugated
• Alternative Names: T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1

Product Description

Anti-Filamin A/FLNA Antibody (monoclonal, 3F8). Tested in IF, IHC, WB applications. This antibody reacts with Human.

Product Properties

• Clonality: Monoclonal
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
• Clone Designation: [3F8]
• Molecular Weight: 281 kDa
• UniProt: P21333
• Form: Lyophilized
• Target: Integrin beta-2

Application Notes

• Application Notes: Application Notes: Western blot, 0.25-0.5µg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human Immunofluorescence, 5µg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Images

IF analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody. Filamin A/FLNA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL mouse anti-Filamin A/FLNA Antibody overnight at 4C. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DyLight488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody. Filamin A/FLNA was detected in paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Filamin A/FLNA Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody. Filamin A/FLNA was detected in paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Filamin A/FLNA Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody. Filamin A/FLNA was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Filamin A/FLNA Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody. Filamin A/FLNA was detected in paraffin-embedded section of human esophageal squamous carcinomar tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Filamin A/FLNA Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody. Filamin A/FLNA was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-Filamin A/FLNA Antibody overnight at 4C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Filamin A/FLNA using anti-Filamin A/FLNA antibody. Filamin A/FLNA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDT