Lamin B1 Recombinant Rabbit Monoclonal Antibody, Clone: [4A3], Unconjugated

Product Overview

• Article Name: Lamin B1 Recombinant Rabbit Monoclonal Antibody, Clone: [4A3], Unconjugated
• Biozol Catalog Number: BYT-ORB704307
• Supplier Catalog Number: orb704307
• Alternative Catalog Number: BYT-ORB704307-100,BYT-ORB704307-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: KLH conjugated synthetic peptide derived from human Lamin B1
• Conjugation: Unconjugated
• Alternative Names: LMNB1_HUMAN, Lamin-B1, LMNB1, LMN2, LMNB, LMN, ADLD, MCPH26,

Product Description

Lamin B1 Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [4A3]
• Molecular Weight: 70 kDa
• UniProt: P20700
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: LMNB1

Application Notes

• Application Dilute: WB=1:10000-50000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:50-200, IF=1:200-800, Flow-Cyt=1ug/Test

Images

Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lamin B1) Monoclonal Antibody, Unconjugated (orb704307) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lamin B1) Monoclonal Antibody, Unconjugated (orb704307) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lamin B1) Monoclonal Antibody, Unconjugated (orb704307) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lamin B1) Monoclonal Antibody, Unconjugated (orb704307) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lamin B1) Monoclonal Antibody, Unconjugated (orb704307) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Lamin B1) Monoclonal Antibody, Unconjugated (orb704307) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Stomach (Mouse) Lysate at 40 ug, Lane 3: Testis (Mouse) Lysate at 40 ug, Lane 4: Cerebrum (Rat) Lysate at 40 ug, Lane 5: Pancreas (Rat) Lysate at 40 ug, Lane 6: Testis (Rat) Lysate at 40 ug, Lane 7: U937 (Human) Cell Lysate at 30 ug, Lane 8: Jurkat (Human) Cell Lysate at 30 ug, Lane 9: Molt-4 (Human) Cell Lysate at 30 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Lamin B1 (orb704307) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 70 kD, Observed band size: 70 kD.

IHC-P analysis of human prostate using Lamin B1 Monoclonal Antibody at 1:200 dilution.

IHC-P analysis of rat stomach using Lamin B1 Monoclonal Antibody at 1:200 dilution.