ATM Recombinant Rabbit Monoclonal Antibody, Clone: [12A], Unconjugated

Product Overview

• Article Name: ATM Recombinant Rabbit Monoclonal Antibody, Clone: [12A], Unconjugated
• Biozol Catalog Number: BYT-ORB704338
• Supplier Catalog Number: orb704338
• Alternative Catalog Number: BYT-ORB704338-100,BYT-ORB704338-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human ATM
• Conjugation: Unconjugated
• Alternative Names: ataxia telangiectasia mutated, AT complementation group A, AT complementation group C, AT complementation group D, AT complementation group E, AT mutated, AT protein, AT1, ATA, Ataxia telangiectasia gene mutated in human beings, ATC, ATDC, ATE, ATM, DKFZp781A0353, Human phosphatidylinositol 3 kinase homolog, MGC74674, Serine protein kinase ATM, T cell prolymphocytic leukemia, TEL1, TPLL.

Product Description

ATM Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Clone Designation: [12A]
• Molecular Weight: 370 kDa
• UniProt: Q13315
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: ATM

Application Notes

• Application Dilute: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200

Images

CRC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ATM (12A)) Monoclonal Antibody, Unconjugated (orb704338) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ATM (12A)) Monoclonal Antibody, Unconjugated (orb704338) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ATM (12A)) Monoclonal Antibody, Unconjugated (orb704338) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATM) Monoclonal Antibody, Unconjugated (orb704338) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATM) Monoclonal Antibody, Unconjugated (orb704338) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATM) Monoclonal Antibody, Unconjugated (orb704338) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ATM) Monoclonal Antibody, Unconjugated (orb704338) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: AGS cell lysate, Lane 2: CRC cell lysate, Primary: Anti-ATM (orb704338) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 370 kD, Observed band size: 370 kD.

IHC-P analysis of Human kidney using ATM Monoclonal Antibody at 1:200 dilution.