ACE2 Mouse Monoclonal Antibody, Clone: [1C4], Unconjugated

Product Overview

• Article Name: ACE2 Mouse Monoclonal Antibody, Clone: [1C4], Unconjugated
• Biozol Catalog Number: BYT-ORB704343
• Supplier Catalog Number: orb704343
• Alternative Catalog Number: BYT-ORB704343-100,BYT-ORB704343-50
• Manufacturer: Biorbyt
• Host: Mouse
• Category: Antikörper
• Application: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from human ACE2 (631-805/805aa)
• Conjugation: Unconjugated
• Alternative Names: ACE-2, ACE 2, Angiotensin converting enzyme 2, ACE related carboxypeptidase, ACEH, Angiotensin converting enzyme homolog, Angiotensin converting enzyme like protein, Angiotensin I Converting Enzyme(peptidyl dipeptidase A) 2, Angiotensin I converting enzyme 2, DKFZP434A014, EC 3.4.17, angiotensin-converting enzyme 2 precursor, ACE2_HUMAN, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2.

Product Description

ACE2 Mouse Monoclonal Antibody

Product Properties

• Clonality: Monoclonal
• Concentration: 1mg/ml
• Clone Designation: [1C4]
• Molecular Weight: 87 kDa
• UniProt: Q9BYF1
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: ACE2

Application Notes

• Application Dilute: WB=1:500-1000, IHC-P=1:400-800, IHC-F=1:400-800, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000

Images

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (orb704343) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (orb704343) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (orb704343) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (orb704343) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ACE2) Monoclonal Antibody, Unconjugated (orb704343) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Recombinant human ACE2 protein, His (HEK293), Primary: Anti-ACE2 (orb704343) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 87 kDa, Observed band size: 110 kDa.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ACE2) monoclonal Antibody, Unconjugated (orb704343) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

IHC-P analysis of Human kidney using ACE2 Monoclonal Antibody at 1:200 dilution.