Phospho-SIRT1 (Ser47) Recombinant Rabbit Monoclonal Antibody, Unconjugated

Product Overview

• Article Name: Phospho-SIRT1 (Ser47) Recombinant Rabbit Monoclonal Antibody, Unconjugated
• Biozol Catalog Number: BYT-ORB704522
• Supplier Catalog Number: orb704522
• Alternative Catalog Number: BYT-ORB704522-100,BYT-ORB704522-25,BYT-ORB704522-50
• Manufacturer: Biorbyt
• Host: Rabbit
• Category: Antikörper
• Application: IF, IHC-Fr, IHC-P, WB
• Species Reactivity: Human
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human SirT1 around the phosphorylation site of Ser47 R(p-S)PG
• Conjugation: Unconjugated
• Alternative Names: SIR2L1, NAD-dependent protein deacetylase sirtuin-1, EC:2.3.1.286, hSIRT1, NAD-dependent protein deacylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1(hSIR2), SirtT1 75 kDa fragment, SIR1_HUMAN,

Product Description

Phospho-SIRT1 (Ser47) Recombinant Rabbit Monoclonal Antibody

Product Properties

• Clonality: Recombinant
• Concentration: 1mg/ml
• Molecular Weight: 81 kDa
• UniProt: Q96EB6
• Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Form: Liquid
• Target: SIRT1

Application Notes

• Application Dilute: WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Application Notes: Modification: Phosphorylated

Images

Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: 293 + CA (-), 2: 293 + CA (+), Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 82 kDa, Observed MW: 110 kDa.

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SIRT1 (Ser47)) Monoclonal Antibody, Unconjugated (orb704522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SIRT1 (Ser47)) Monoclonal Antibody, Unconjugated (orb704522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SIRT1 (Ser47)) Monoclonal Antibody, Unconjugated (orb704522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SIRT1 (Ser47)) Monoclonal Antibody, Unconjugated (orb704522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SIRT1 (Ser47)) Monoclonal Antibody, Unconjugated (orb704522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Phospho-SIRT1 (Ser47)) Monoclonal Antibody, Unconjugated (orb704522) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue: Human gastric adenocarcinoma, Section type: Formalin-fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab, dilution: 1:200, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: Anti-Rabbit and Mouse, Polymer HRP (Ready to use), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb704522.

IHC-P analysis of human tonsil using Phospho-SIRT1 Ser47 Monoclonal Antibody at 1:200 dilution.