Human TNFSF13B protein

Product Overview

• Article Name: Human TNFSF13B protein
• Biozol Catalog Number: BYT-ORB705331
• Supplier Catalog Number: orb705331
• Alternative Catalog Number: BYT-ORB705331-1,BYT-ORB705331-100,BYT-ORB705331-20
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: B lymphocyte stimulator (BLyS) (B-cell-activating factor) (BAFF) (Dendritic cell-derived TNF-like molecule) (TNF- and APOL-related leukocyte expressed ligand 1) (TALL-1)

Product Description

This Human TNFSF13B protein spans the amino acid sequence from region 134-285aa. Purity: Greater than 93% as determined by SDS-PAGE.

Product Properties

• Molecular Weight: 46.6 kDa
• UniProt: Q9Y275
• Buffer: Lyophilized from a 0.2 µm sterile filtered PBS, 6% Trehalose, pH 7.4
• Source: Homo sapiens (Human)
• Purity: Greater than 93% as determined by SDS-PAGE.
• Form: Lyophilized powder
• Sequence: AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

Application Notes

• Application Notes: Biological Origin: Homo sapiens (Human). Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 10 µg/ml can bind human BCMA , the EC50 of human TNFSF13B protein is 221.3-298.6 ng/ml.Human SIRPA protein His/Myc tag captured on COOH chip can bind Human CD47 protein Fc tag with an affinity constant of 19.1 nM as detected by LSPR Assay.Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 µg/ml can bind TNFRSF13C, the EC50 is 9.943-15.72 ng/ml. Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference

Images

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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 10 µg/ml can bind human BCMA, the EC50 of human TNFSF13B protein is 221.3-298.6 ng/ml.

Human TNFSF13B protein Fc tag captured on COOH chip can bind Human BCMA protein Fc tag with an affinity constant of 39 nM as detected by LSPR Assay.

Measured by its binding ability in a functional ELISA. Immobilized TNFSF13B at 2 µg/ml can bind TNFRSF13C, the EC50 is 9.943-15.72 ng/ml.

The purity of TNFSF13B was greater than 90% as determined by SEC-HPLC