Human PDCD1 protein

Product Overview

• Article Name: Human PDCD1 protein
• Biozol Catalog Number: BYT-ORB705336
• Supplier Catalog Number: orb705336
• Alternative Catalog Number: BYT-ORB705336-1,BYT-ORB705336-100,BYT-ORB705336-20
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: (Protein PD-1) (hPD-1) (CD279)

Product Description

This Human PDCD1 protein spans the amino acid sequence from region 25-167aa. Purity: Greater than 93% as determined by SDS-PAGE.

Product Properties

• Molecular Weight: 18.2 kDa
• UniProt: Q15116
• Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
• Source: Homo sapiens (Human)
• Purity: Greater than 93% as determined by SDS-PAGE.
• Form: Lyophilized powder
• Sequence: LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ

Application Notes

• Application Notes: Biological Origin: Homo sapiens (Human). Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 µg/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 µg/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml. Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference

Images

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 µg/ml can bind Anti-PD-1 recombinant antibody, the EC50 of human PD-1 protein is 6.087-7.854 ng/ml.

②Measured by its binding ability in a functional ELISA. Immobilized PD-1 at 2 µg/ml can bind Nivolumab, the EC50 of human PD-1 protein is 9.713-12.39 ng/ml.