Human CD40LG protein

Product Overview

• Article Name: Human CD40LG protein
• Biozol Catalog Number: BYT-ORB705340
• Supplier Catalog Number: orb705340
• Alternative Catalog Number: BYT-ORB705340-1,BYT-ORB705340-100,BYT-ORB705340-20
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Alternative Names: (CD40-L)(T-cell antigen Gp39)(NF-related activation protein)(TRAP)(Tumor necrosis factor ligand superfamily member 5)(CD154)(sCD40L)

Product Description

This Human CD40LG protein spans the amino acid sequence from region 13-261aa. Purity: Greater than 87% as determined by SDS-PAGE.

Product Properties

• Molecular Weight: 44.5 kDa
• UniProt: P29965
• Buffer: Lyophilized from a 0.2 µm sterile filtered PBS, 6% Trehalose, pH 7.4
• Source: Homo sapiens (Human)
• Purity: Greater than 87% as determined by SDS-PAGE.
• Form: Lyophilized powder
• Sequence: MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL

Application Notes

• Application Notes: Biological Origin: Homo sapiens (Human). Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 µg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody, the EC50 is 2.353-3.232 ng/ml. Application Notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference

Images

orb705340

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized CD40L at 2 µg/ml can bind Anti- CD40L Rabbit Monoclonal Antibody, the EC50 is 2.353-3.232 ng/ml.

Measured by its binding ability in a functional ELISA. Immobilized CD40 at 2 µg/ml can bind CD40L, the EC50 is 3.112-3.858 ng/ml.

Human CD40 protein hFc tag captured on COOH chip can bind Human CD40L protein hFc and Flag tag with an affinity constant of 2.06 nM as detected by LSPR Assay.