N Cadherin/CDH2 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:
BYT-ORB738374
Supplier Catalog Number:
orb738374
Alternative Catalog Number:
BYT-ORB738374-100
Manufacturer:
Biorbyt
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, FC, ICC, IF, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
E.coli-derived human N Cadherin/CDH2 recombinant protein (Position: E170-E266).
Conjugation:
Unconjugated
Alternative Names:
Protein phosphatase 1D, Protein phosphatase 2C isoform delta, PP2C-delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PPM1D, WIP1
Anti-N Cadherin/CDH2 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Clonality:
Polyclonal
Concentration:
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Application Notes: Western blot, 0.25-0.5µg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human Immunofluorescence, 5 µg/ml, Human Flow Cytometry (Fixed), 1-3µg/1x106 cells, Human ELISA, 0.1-0.5µg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Flow Cytometry analysis of HEPG2 cells using anti-N Cadherin/CDH2 antibody. Overlay histogram showing HEPG2 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-N Cadherin/CDH2 Antibody (1 µg/1x10 6 cells) for 30 min at 20C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x10 6 cells) was used as secondary antibody for 30 minutes at 20C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10 6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody. N Cadherin/CDH2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-N Cadherin/CDH2 Antibody overnight at 4C. Biotin conjugated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using DyLight488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody. N Cadherin/CDH2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-N Cadherin/CDH2 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody. N Cadherin/CDH2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-N Cadherin/CDH2 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody. N Cadherin/CDH2 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-N Cadherin/CDH2 Antibody overnight at 4C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of N Cadherin/CDH2 using anti-N Cadherin/CDH2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-N Cadherin/CDH2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4C, then washed with TBS-0.1%
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