Bovine Collagen Type I protein, Unconjugated

Product Overview

• Article Name: Bovine Collagen Type I protein, Unconjugated
• Biozol Catalog Number: BYT-ORB750375
• Supplier Catalog Number: orb750375
• Alternative Catalog Number: BYT-ORB750375-500
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: ELISA, IHC, SDS-PAGE, WB
• Conjugation: Unconjugated
• Alternative Names: Type I collagen protein, collagen 1 protein, bovine collagen protein, Alpha 1 type I collagen protein, Alpha 2 type I collagen protein, COL1A1 protein, COL1A2 protein, Collagen alpha-1(I) chain protein, Collagen alpha-2 (I) chain protein

Product Description

Bovine Collagen Type I protein

Product Properties

• Concentration: 1 mg/ml
• NCBI: 001029211
• UniProt: P02453
• Buffer: 0.01% (w/v) Sodium Azide
• Source: Bovine
• Purity: Bovine Collagen Type 1 has been prepared from Bovine Placenta and is chromatographically and immunologically pure. Bovine Collagen Type 1 reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).
• Form: Liquid (sterile filtered)
• Target: COL1A1

Application Notes

• Application Dilute: IF: 1:100-1:500, WB: 1:1000
• Application Notes: Biological Origin: Bovine. Application Notes: Bovine Collagen Type I purified protein standard is used as a control for SDS-PAGE, Western Blot, ELISA, immunoprecipitation, and for other immunological assays. Specific conditions should be optimized by user. Collagen type I is recognized by type specific Anti-Collagen antibodies that recognize a native three-dimensional structure

Images

Aggf1 interacts with and modulates SMAD7 activity. (D) Primary HSCs were infected with Ad-V or Ad-AGGF1 and then transfected with either siRNA targeting SMAD7 or scrambled siRNA (SCR). Expression of fibrogenic genes was examined by Western and qPCR (not shown).

Aggf1 suppresses liver fibrosis following injury in mice. (B) C57/BL6 mice were injected via tail vein adenovirus carrying either Aggf1 expression vector (Ad-AGGF1) or an empty vector (Ad-V) followed by CCl4 injection to induce liver injury. Expression of fibrogenic genes was examined by Western and qPCR (not shown).

C57/BL mice were injected peritoneally with chaetocin (25 mg/kg) or DMSO 2 days prior to the LAD procedure. The mice were sacrificed 4 weeks after the surgery. (B) Expression of pro-fibrogenic genes was examined by Western blotting and qPCR (not Shown).

SDS-PAGE Results of Bovine Collagen Type I. Lane 1: Bovine Collagen Type I -Reduced [10 µg]. Lane 2: Opal Prestained Molecular Weight Marker Lane 3: Bovine Collagen Type I -Non-Reduced [10 µg]. 4-20% Gel, Coomassie Stained.