Human Collagen Type I protein, Unconjugated

Product Overview

• Article Name: Human Collagen Type I protein, Unconjugated
• Biozol Catalog Number: BYT-ORB750381
• Supplier Catalog Number: orb750381
• Alternative Catalog Number: BYT-ORB750381-500
• Manufacturer: Biorbyt
• Category: Proteine/Peptide
• Application: ELISA, IHC, SDS-PAGE, WB
• Conjugation: Unconjugated
• Alternative Names: Type I collagen protein, collagen 1 protein, human collagen protein

Product Description

Human Collagen Type I protein

Product Properties

• Concentration: 1.0 mg/mL
• NCBI: 000079
• UniProt: P02452
• Buffer: 0.01% (w/v) Sodium Azide
• Source: Human
• Purity: Human Collagen type I has been prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. Human Collagen type I reacts with anti-Collagen Type I. Reaction of Human Collagen type I with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross-reactivity was detected by ELISA).
• Form: Liquid (sterile filtered)
• Target: COL1A1/COL1A2

Application Notes

• Application Dilute: IF: 1:100-1:500, WB: 1:1000
• Application Notes: Biological Origin: Human. Application Notes: Human Collagen type I purified protein standard is used as a control in ELISA and western blot. Collagen type I is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure

Images

Glycated collagen 1 regulates adipocyte insulin signaling and Rho and AGE scavenger receptor expression. Preadipocytes from human VAT were differentiated into mature adipocytes in 2D culture + /- unglycated or glycated recombinant human collagen 1 (UC1, GC1), then studied with glucose uptake assay or RT-qPCR. (a) Glucose uptake in non-stimulated (basal) or insulin-stimulated conditions. Ordinate: mean glucose uptake measured by 3H-2D-glucose in cell lysates (cpm) normalized to cell lysate protein concentration (mg/ml), bars with different letters indicate P < 0.050, n = 15 NDM, 15 DM subjects. (b) Gene expression studied with RT-qPCR. Ordinate: mean fold difference in transcript level in GC1 arm relative to UC1 arm referent = 1, *P < 0.050, comparing transcript levels in GC1 arm vs. UC1 arm, n = 12 NDM, 12 DM subjects.

SDS-PAGE Results of Human Collagen Type I. Lane 1: Human Collagen Type I Reduced (5.0 µg). Lane 2: Opal PRestained Molecular Weight Marker. Lane 3: Human Collagen Type I Non-Reduced (5.0 µg). 7.5% Gel, Coomassie Stained. Observed MW: Type I trimer, Type I dimer alpha1, alpha2, Type I monomer alpha1, alpha2.

Western blot of Human Collagen Type I. Lane 1: Human Collagen Type 1. Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1000 overnight at 4C. Secondary antibody: DyLight(TM) 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms.